Journal: Frontiers in Pediatrics
Article Title: Expression and Possible Role of Silent Mating Type Information Regulation 2 Homolog 1 in Post-necrotizing Enterocolitis Stricture in vivo and in vitro
doi: 10.3389/fped.2022.836128
Figure Lengend Snippet: The influence of Sirtuin1 or Silent mating–type information regulation 2 homolog-1 (SIRT1) on the expression of epithelial–mesenchymal transition (EMT) marker proteins in intestinal epithelial cells (IEC). (A) Immunofluorescence staining of E-cadherin protein in control negative (NC) groups and siRNA-SIRT1 group cells (10 × 10). (B) Immunofluorescence staining of Vimentin protein in NC groups and siRNA-SIRT1 group cells (10 × 10).
Article Snippet: The CCK8 kit (Tongren Chemical, Japan), the vascular endothelial growth factor (VEGF)-ELISA kit (Elabscience, China), the gene sequence of siRNA-SIRT1 (Invitrogen Company, USA), the epithelial–mesenchymal transition (EMT) detection marker E-cadherin (USA abcam, ab40772), and Vimentin (USA abcam, ab92547) were used.
Techniques: Expressing, Marker, Immunofluorescence, Staining